Search for: [Abstract = "phospholipid levels were measured by means of enzymatic colorimetric tests. Individual bile acids were measured using high performance liquid chromatography after prior extraction. Total bile acid concentration was obtained by summing up the individual bile acid concentrations. On the basis of individual lipid concentrations in bile, the cholesterol saturation index was calculated for each bile sample, using Carey tables. Regarding the quantitative analysis, the ICCs were qualified as\: c\-kit\-immunopositive and tryptaseimmunonegative concurrently and posing nucleus \(to eliminate other non\-cellular structures\). Interstitial cells of Cajal had a fusiform shape predominantly, 40 \- 60 um length, and in some preparations its branches were also visualized. ICCs were seen in the whole area of the gallbladder including its fundus, body and neck. These cells were identified almost exclusively within the gallbladder muscle wall and were oriented parallel to the smooth muscle cells. The following subpopulations of the ICCs were spotted\: ICC\-IM \(ang. intramuscular ICC\) localized between smooth muscle fibres forming one muscle bundle and ICC\-IB \(ang. interbundle ICC\) localized within connective tissue separating smooth muscle bundles. Interstitial cells of Cajal typically appeared singly, sometimes in small clusters consisting of 2\-3 cells, but they did not form a gl"]

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