Search for: [Abstract = "oth D\-hydantoinases were compared and substrate specificity towards all 21 obtained \(R,S\) phenyl ring\-substituted 5 benzylhydantoine derivatives were determinated. Reaction rate constant and substrate half\-life in different pH conditions were also estimated. High pressure liquid chromatography was used for determinating enantiomeric ratio \(%ee\) of the obtained D\-phenylalanine derivatives. The enantioseparations were performed using a ChiroSil \(RCA\+\) column containing the chiral stationary phase prepared by a covalent trifunctional bonding of \(\+\) or\(\-\)\-\(18\-Crown\-6\)\-tetracarboxylic acid. For the first time the enantioselectivity of D\-hydantoinase, recombinant, immobilized from E. coli towards novel phenyl ring\-substituted 5 benzylhydantoine derivatives was successfully evaluated. The enantioseparations of a few obtained D\-phenylalanine derivatives using Capillary Electrophoresis were also performed. In order to accomplish enantioseparation the optical isomers of amino acids have to come into contact with a chiral environment to form two diastereomeric complexes. In the present work were used highly sulfated cyclodextrins \(HS\-CDs\) as a chiral selectors. To determine enantiomeric purity, enantiomeric ratio \(%ee\) for each examined compound was calculated. Several obtained D\-phenylalanine derivatives possess structural similarity to the described glutamate receptor active phenyla"]

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