Search for: [Abstract = "ons of the scaffolds and culture treatments. Compared to silica\-rich S2\-PLGA composites, hBMSC grown on calcium\-rich A2\-PLGA scaffolds were overall less responsive to rhBMP\-2 or DEX and the osteoinductive properties of these A2\-PLGA scaffolds seemed to be partially dependent on activation of BMP signaling in untreated hBMSCs. Our composite scaffolds, specifically S2\-PLGA also enhanced hBMSCs osteogenesis under a single, 2\-hour session of unidirectional, steady, 2.5 ml\/min flow perfusion stimulation and rhBMP\-2 treatment. We also cultured hBMSCs on bioinert, porous, gelatine\-coated, polyurethane scaffolds in the presence of the osteogenic supplements and stimulated them with a single 2\-hour session of media flow perfusion at either early or late 3D culture stages. Some of the cells also were pre\-treated in monolayers \(2D cultures\) with the osteogenic supplements to advance cell differentiation and followed by the osteogenic 3D cultures. These experiments were performed in order to characterize cell differentiation stage effects under flow perfusion stimulation. We noted that a single, short, unidirectional and steady media perfusion in hBMSC 3D cultures can significantly enhance expression of bone\-related transcription and growth factors along with the enhancement of matrix components \(collagen and mineral\) production. However, it is the most effective when cells reach the pre\-o"]

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