Search for: [Abstract = "of proteins significant for cancer growth \(VEGF, MMP\-2 and 9, MIA\) and the potential of PPAR ligands for induction of programmed cell death, i.e. apoptosis, was investigated. The study was conducted on four cell lines derived from different stages of melanoma development\: WM35 – from primary site, horizontal growth phase \(RGP\), WM9 – from metastatic site in lymph nodes, WM239A – from metastatic site in the skin, and A375P – from malignant solid tumor in lungs. The study employed commonly accepted analytical techniques, including classical biochemical methods \(spectrophotometry, spectrofluorymetry, centrifugation, in native and denaturing condition electrophoresis of proteins, electrophoresis of DNA zymography, Western blot, chromatography\), cell biology techniques \(monolayer cell cultures, staining techniques, microscopic analysis\) and molecular biology tools \(DNA and RNA isolation, RT\-PCR, electrophoresis of PCR products, lipotransfection of cells using specific vectors, transcriptional activity testing, gel shift assay\). It was shown that melanoma cells expressed all three forms of active PPARs. PPAR ligands at concentrations used in these experiments \(ciglitazon \- 5, 10, 15 μM, fenofibrat \- 5, 10, 15 μM, karbacyklin \- 0.1, 1.0, 5.0 μM, linoleic acid \- 15, 20, 30 μM and 9\-cis\-retinoic acid \- 0.1, 0.3, 1.0\) decreased proliferation of melanoma cells, however, these changes"]

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