Search for: [Abstract = "ny catechins. After 24 hours of incubation all cultures were harvested and mitochondria were isolated. In mitochondrial protein extract PDK activity as well as expression of PDK\-2 isoenzyme were assayed. PDK activity was assayed spectrophotometrically by the rate of ATP\-dependent inactivation of PDH complex and activity was calculated as the first order rate constant. Expression of PDK\-2 protein was assayed by Westem blott analysis. The results of study presents adverse correlation between PDK activity and concentration of tested polyphenols. It was demonstrated that addition of all tested polyphenols to culture medium remarkably decreased PDK activity comparing with control group. The degree of reduction of PDK activity strongly depends on the dose and also on the structure of particular polyphenolic compounds. It was also proved that ethanol increases PDK activity, opposite polyphenols. In conclusion, it is conceivable that chosen polyphenols affecting PDK activity modify PDH complex activity and in consequence exert an effect on glucose and lipid metabolism. Polyphenols may possibly act via PP ARα system on expression of genes of PDK in rat's hepatocytes. On the basis of above polyphenolic compounds may regulate activity of glucose and lipid metabolism."]

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