Search for: [Abstract = "nts were performed to monitor chimerism of transplanted mice by means of sry gene detection by PCR and real time PCR. Furthermore, 60 C57BL\/6 syngeneic transplantations and 8 C3H.He syngeneic transplantations were performed to provide control groups \(without GvHD\). All the transplantations were preceded by total body irradiation. Following transplantation, mice were weighted and physically examined by looking for changes in skin and posture. Peripheral blood morphology assessment and flow cytometry analysis of peripheral blood lymphocytes were performed for some animals on days \+17, \+31 and \+45. Additionally, detection of TREC\-positive cells was performed on day \+45. Histological examination of liver, skin, colon and spleen was performed on day \+8 and \+16. In mice transplanted with allogeneic cells evident symptoms of GvHD were observed in physical examination \(weight loss, skin desquamation, hunching\), in analysis of peripherial blood lymphocytes \(lymphopenia, increased expression of CD69 activation antigen on CD3\+CD4\+ and CD3\+CD8\+ cells, deficiency of TREC\-positive cells\) and in histological picture of examined organs. The most important difference between C3\-\/\- and C3\+\/\+ allogeneic graft recipient populations was significantly better survival of wild type animals \(HR 1,6\; p=0,0273\). Presence of valid complement system was also associated with beneficial effect on weight of t"]

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