Search for: [Abstract = "ntrol group consisted of 25 consecutive patients operated electively for pancreatic head tumours, with no pre\- and intraoperative signs of cholelithiasis and jaundice. The study has been conducted, with the Jagiellonian University Bioethical Commitee's consent, according to the Declaration of Helsinki guidelines. The primary aim of this study was to identify and determine the quantity and localization of the interstitial cells of Cajal within the gallbladder muscle wall in patients suffering from cholelithiasis, and in patients from the control group. The second goal was to measure concentrations of the lipid constituents in bile taken from patients in both groups i.e. cholesterol, phospholipids and bile acids. Identification of ICCs within the transverse sections of the gallbladder wall, was performed by means of the double immunofluorescence technique using anti c\-kit and anti tryptase antibodies to better distinguish ICCs from mast cells. Assessment of the quantity and distribution of the ICC in the gallbladder was carried out using immunofluorescence microscopy conjoined with an image analysis system \(ProgRes Capture Pro v.2.8, ProgRes C12Plus camera \(Jenoptik\)\). Gallbladder bile samples were taken intraoperatively to be subsequently analyzed for cholesterol, phospholipid, as well as, total and individual bile acid concentrations. Cholesterol and"]

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