Szukana fraza: [Abstrakt = "nature and – in recent years more frequently – towards medicinal mushrooms. Aims of study The aim of this dissertation was to scientifically approach the question of whether Fomitopsis officinalis – an arboreal medicinal mushroom, known for thousands of years for its health–promoting properties – is a promising target for such pursuits. Due to its status of an endangered species, any attempt to use Fomitopsis officinalis in the prevention of civilization diseases must be based on material obtained in vitro. This approach opens a possibility of enrichment \(fortification\) of the material during cultivation. Therefore, the second goal of this study was to investigate the potential impact of fortification with zinc or magnesium salts on the composition and properties of Fomitopsis officinalis. Methods In order to answer the above questions, the following methods were used\: 1\) mycochemistry analyses\: HPLC – to measure the levels organic compounds, FAAS – to measure the levels of bioelements in the obtained material\; 2\) in vitro tests\: DPPH – to investigate antioxidant activity, MTT – to study anti–cancer activity in the lung \(A549\), prostate \(DU 145\) and skin \(A375\) cancer cell lines, and a mouse macrophage cell line RAW 264.7 – to examine the effect of the material on inflammation\; 3\) molecular biology methods\: PCR and sequencing – to confirm the iden"]
