Search for: [Abstract = "lecular techniques\: PCR\-SSCP reactions, which allowed to detect mutations. and to loca te them in a proper ex on of the investigated gene were used in this work. Statistical analysis was done using computer program\: Statistica for Windows 6.0. results Bands pattem analysis revealed presence of CDH I gene mutations in 13% of investigated gastric cancers. When only so\-called diffuse and mixed types were taken under consideration, the mutations were present in 19% of investigated cases. Disturbances in expression of E\-cadherin protein were detected immunohistochemicaly in about 20% \( 17 cases\) and of B\-catenin protein in about 19% \(16 cases\). Statistically significant correlation between the presence ofEcadherin gene mutation and disturbances in E\-cadherin protein expression was found. It was noticed that a part of sporadic, advanced gastric cancers with detected mutation in CDH1 gene do not show abnormal expression of the E\-cadherin protein. Some cases with unmutated E\-cadherin gene showed a deranged protein expression, most probably due to the epigenetic factors. Deranged expression of proteins belonging to the ECCU identifies a gro up of patients with poorer overall survival. The E\-cadherin mutation however, does not have any prognostic impact. In the present study it was possible to confirm the value oftraditional prognostic factors\: the lymph node status, distant metast"]

Number of results: 1