Search for: [Abstract = "lanine or phenylglycine derivatives. Using Amberlite 120 H\+ ion\-exchange resin and next liophilisation process were purified and isolated proper D\-amino acids from the reaction mixtures. The compounds were next examined for their activity against glutamate receptors. Reported was also an enzymatic route which enables the subsequent hydrolysis of N\-cabamoyl\-D\-amino acid to the corresponding D\-phenylalanine derivative, mediated by N\-carbamoylase \(rN\-Kar\). Plasmid pAH71 containing the N\-carbamoylase gene used in this study was kindly donated by Prof. Yun\-Peng Chao from the Department of Chemical Engineering, Feng Chia University, Taiwan. For inducing N\-carbamoylase gene 1 mM of IPTG \(Isopropyl β\-D\-1\-thiogalactopyranoside\) was added to transformed, recombinant Escherichia coli strains. The presence of N\-carbamoylase was confirmed by SDS\-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis. Recombinant, inducted E.coli strains were used for biotransformation of the obtained previously N\-carbamoyl\-D\-amino acids to the corresponding D\-amino acids. The reactions were monitored by capillary electrophoresis. In this work capillary electrophoresis was also used as a tool for DNA separations. The comparison of CE DNA separation method with conventional agarose gel electrophoresis was also performed."]

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