Search for: [Abstract = "hase \(cPGES\), fatty acid\-binding protein 4 \(FABP4\), toll\-like receptor 4 \(TLR4\), glucose type 4 receptor \(GLUT\-4\) and the cannabinoid2 receptor \(CB2\) were determined by Western blotting. Gene expression of phospholipase A2 \(Pla2g4a\) and prostaglandin E2 synthase \(Ptgs2\) was analyzed by real\-time qPCR. After the activation of EPA and IF, a significant decrease in COX\-2, cPGES, and TLR4 proteins were observed. Incubation of cells with EPA and IF resulted in repression of Ptgs2 and increased expression of the Pla2g4a gene. A significant increase in CB2 protein in adipocytes incubated simultaneously with EPA and IF was noted. The obtained results indicate the anti\-inflammatory properties of EPA. The activation of the GLUT4 receptor by EPA suggests a unique role for this fatty acid in regulating adipocyte metabolism and preventing insulin resistance. In another experiment, pre\-adipocytes and adipocytes were supplemented with 40 μmol EPA, 40 μmol DHA, and 1 nmol of Resolvin D1 \(RvD1\) and activated with 1 μmol of benzo \(a\) pyrene \(BaP\). Identification and quantification of the isoprostanes 8\-iso\-prostaglandin F2α \(8\-isoPGF2α\), 8\-isoPGF3α, prostaglandin F2α \(PGF2α\), and PGF3α were performed using ultra\-high\-performance liquid chromatography UHPLC UltiMate 3000 RS system coupled with a mass spectroscope and a transfer time analyzer. The highest amounts of 8\-isoPF2α, 8\-isoPGF3α, and PGF"]

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