Search for: [Abstract = "growth, viability, metabolic activity and ability to differentiate into osteoblastic lineage of human ASCs from subcutaneous fat tissue on nanoporous TiO2 \(with the pore diameters of 80 and 108 nm\). Methods\: A three\-step electrochemical anodization process in glycerol\-based electrolyte with fluoride ions was performed to produce a nanoporous oxide coat on Ti surfaces. Surface characterization of the nanostructured oxide layer and cell morphology was carried out with electron scanning microscopy. Human adipose derived stem cells were isolated from subcutaneous fat tissue of healthy donors and cultured on titanium samples for 21 days. In vitro experiments to assess cell growth \(BrdU incorporation test\), activity \(ATP monitoring and total protein concentration\) and viability \(LDH release test\) were carried out. Cell differentiation was determined by alkaline phosphatase activity, gene expression \(BGLAP, SP7, TBX3, DLX5, RUNX2, ALPL, BMP2K, COL1A1, SPP1, NOTCH1, SPARC\) and alizarin red staining. Results\: A nanoporous layer of TiO2 was successfully produced on Ti surfaces using a relatively simple and economic technique. The nanostructured oxide coat significantly induced the osteo\-differentiation of ASCs after 21\-day in vitro culture in comparison with conventional titanium. There were no powerful differences between two nanoporous \(80 nm vs 108 nm\) surfaces. Conclusion\: Our"]

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