Search for: [Abstract = "entration of 0.63 mg\/200 μl \(3.15 mg\/1 ml\). In addition, the cytotoxic effect was visualized by optical microscopy and SEM. . Using LIVE\/DEAD double staining and fluorescence microscopy, the cytotoxicity of the functionalized surface was assessed. A549 cell line was used in a cytotoxicity investigation, and the functionalized surfaces had no discernible impact on cell death. Standard strains of Staphylococcus aureus ATCC 25923 \(representative of Grampositive bacteria\) and Escherichia coli ATCC 25922 \(representative of Gram\-negative bacteria\) were used to check the surface for bacterial adhesion. The use of gentamicin nanoparticles significantly reduces the adhesion of bacterial colonies on the surface of the tracheostomy tube for S. aureus from 6E\+05 CFU\/mL to 5E\+03 CFU\/ml and for E. coli from 1.655\+05 CFU\/ml to 2E\+01 CFU\/ml. The result is statistically significant \(p<0.001\). Conclusions In order to enhance the qualities of PVC surfaces for tracheostomy patient applications, a series of surface chemistry and microbiology investigations were used in this PhD thesis. The chosen techniques enabled the creation of a novel functionalized PVC surface with tissuefriendly and antibacterial characteristics. According to the \"green chemistry\" tenets, the research was conducted using straightforward, more affordable, and ecologically benign approaches."]
