Search for: [Abstract = "employing four non\-cross\-reacting antigens \- DNP\-BSA, MBP, OVA or OX\-Ig \- showed that suppression induced via EC immunization with an antigen is antigen non\-specific.Further experiments, in a model of “transfer in” and adoptive cell transfer of CHS, demonstrated that suppressive activity could be transferred by cells isolated from the axillary and inguinal lymph node cells, spleen and thymus of EC\-immunized mice. The lowest number of EC\-induced Treg cells which could effectively inhibit Tc1 CD8\+\-dependent CHS was 1.35x107 unseparated LNC and only 2x106 of purified Treg cells. Finally we showed that transfer of EC\-induced Treg cells either at the time of immunization or challenge significantly suppressed CHS response.The phenotype of the Treg lymphocyte was found using the negative selection experiment with monoclonal antibodies and rabbit complement, FACS sorting and TCRδ\-\/\- \(mice lacking T lymphocytes with the TCRγδ receptor\), CD1d\-\/\- \(mice lacking NKT lymphocytes DC1d dependent\) and β2m\-\/\- \(mice lacking CD8 lymphocytes\) mice. These data suggested that EC immunization with DNP\-BSA induced a population of Treg cells with a TCRαβ\+CD4\+CD25\+Foxp3\+ phenotype.In the final section of this dissertation, the mechanism of suppression induced via EC application of DNP\-BSA was discussed. Four different approaches were used\: the expression of cytokine production by lymph nodes, the influe"]

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