Search for: [Abstract = "ee different methods of SNAI1 expression modulation\: knockout with GE tools and knockdown with shRNA technology. These results will be used in further studies investigating the role of SNAI1 in RMS. Induced pluripotent stem \(iPS\) cells with their ability to differentiate into three germ layers constitute a perfect tool to study human embryo development processes such as myogenesis. Currently, many protocols describing the differentiation of iPS cells into myogenic cells have been described in the literature. They differ in many aspects, such as media components, including signaling modulators, feeder layer constituents, and duration of culture. The aim of the current study was to verify and compare, side by side, three different protocols of skeletal muscle differentiation with the characteristics of the obtained cells. Therefore, iPS cells were differentiated using protocols previously described in the literature. Protocol I was based on embryonic bodies differentiation induction, ITS addition, and selection with adhesion to collagen I type. Protocol II started with strong myogenic induction at the embryonic bodies step with BIO, forskolin, and bFGF. Protocol III utilizes monolayer cell culture in three special media, leading to WNT activation and TGF\-β and BMP signaling inhibition. Myogenic induction was confirmed by the hierarchical expression of myogenic"]

Number of results: 1