Search for: [Abstract = "e physicochemical characterization of the tested systems. 3\) Evaluation of the effectiveness of surface functionalization and its impact on the growth of bacterial biofilm using reference strains of Staphylococcus aureus and Escherichia coli. Evaluation of the cytotoxicity of obtained biomaterials using the 549\-cell line. Materials and Methods The period of collecting clinical material lasted from June 2018 to March 2020. The study was approved by Jagiellonian University Bioethical Committee no. 1072.1612.153.2019. Patients for the study were recruited at the 5th Military Hospital with Polyclinic in Krakow. The study involved 10 women and 35 men. All patients were older than 18 years with a mean age of 67.9 years \(range 36\-89 years\) and had used a tracheostomy tube for at least 24 hours. Conventional microbiological cultures were made from the collected material, along with the assessment of drug resistance of bacterial strains. Material for experimental research in the field of physiochemistry consisted of sterile packed discs with dimensions of 15 mm in diameter and 2 mm in thickness, cut from the same material as tracheostomy tubes made of poly\(vinyl chloride\). The surface of the discs was functionalized using oxygen plasma, and then formed and immediately sonochemically deposited gentamicin nanoparticles. The resulting surfaces were characterized by AFM, W"]
