Search for: [Abstract = "e by standard organic method or with the use of SwabDNA kit. DNA concentration was measured with fluorimetric method. Then complete MC1R exon was amplified. PCR products were purified and subjected to cycle sequencing. The specific nucleotide positions of the MC1R gene were analysed using minisequencing method. To analyse A8818G polymorphism we used PCR primers allowing to amplificate gene fragment containing this position. Results and conclusions At least one MC1 R allelic variant was present in 70% of the subjects from a control group, which confirms that this gene is highly polymorphic in a Polish population. Allele G frequency was 3% in the control group. We confirmed MC1R gene significant influence on presence of fair phenotype. Subjects with \"major function mutations\"\-R significantly more frequently had phototype I or II with medium or high freckle count and red or blond hair colour. The presence of a variant allele correlated with the mole count\; R151C, R160W, 0294H variants were more common in subjects without or with fewer number of nevi. Our results together with data from the literature neglect MC1R influence on melanoma risk through the number of moles. We did not found correlation between ASIP variants and phenotypic characteristics of the studied subjects. The MC1R genotype was associated with cutaneous melanoma risk. This correlation was the most significant"]

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