Search for: [Abstract = "chronic, low\-grade inflammation, and an increase in energy intake while reducing energy expenditure. Therefore, this dissertation used the in vitro model of adipocytes \(differentiated 3T3\-L1 cells\) and enterocytes \(Caco\-2 cells\). Fully differentiated 3T3\-L1 adipocytes have the most morphological and biochemical features typical of adipose tissue cells in vivo. Due to its morphological and functional analogy, the Caco\-2 lineage is considered the in vivo counterpart of enterocytes of the small intestine. Therefore, it is a suitable model for bioavailability studies. This study aimed to determine the effect of n\-3 fatty acids, EPA, and DHA on 3T3\-L1 fibroblasts and Caco\-2 intestinal epithelial cells, activated by inflammatory factors, and thus targeted programming of fatty acid metabolism in inflammatory conditions. No cytotoxic effects or apoptosis was observed in 3T3\-L1 cells before and after differentiation into adipocytes incubated with EPA or DHA, metformin \(MET\), and lovastatin \(LOVA\) in Caco\-2 cells after supplementation with EPA and DHA and activation with inflammatory factors. Murine embryonic 3T3\-L1 fibroblasts were differentiated into adipocytes. Cells were incubated with 50 μmol EPA for 48 hours and then activated with lipopolysaccharide \(LPS\) or tumor necrosis factor\-α \(TNF\-α\). Cycloxygenase\-2 \(prostaglandin E2 synthase, Ptgs2, COX\-2\), cytosolic prostaglandin E2 synt"]

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