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Search for: [Abstract = "chemical composition and bioactivity \(experimental scaffolds based on a biodegradable poly\-L\-lactide\-D\-glycolide \(PLGA\) copolymer and two types of bioactive glasses\: S2 or A2 or bioinert scaffolds made of polyurethane \(PU\) coated with gelatin\) and the assessment of hBMSC mechanical stimulation in flow perfusion bioreactor in these 3D cultures. The research project presented was focused on the selection and optimisation of the in vitro culture conditions, most effectively promoting differentiation of human BMSCs towards a bone tissue. The experiments were carried out both in static conditions or under media flow perfusion stimulation in hBMSC cultures established on PLGA\- or PU\-based scaffolds. The cells were either cultured in a standard growth medium or stimulated with appropriate osteoinductive supplements\: AA\-2P, DEX, BGP and rhBMP\-2. \; BGs\-PLGA composites have exhibited high bioactivity, manifesting with the formation of calcium phosphate \(apatite\) precipitates inside the pores of the scaffolds. We also have characterised mechanical properties of both composites and shown they promoted superior hBMSC attachment to the scaffolds surface and stimulated the expression of several osteogenic markers in hBMSC compared to cells grown on unmodified PLGA. We concluded there were also marked differences in the response of hBMSCs to composite scaffolds, depending on chemical compositi"]

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