Search for: [Abstract = "ay suggest that, similar to mucosal exposure, EC immunization could induce peripheral tolerance when special conditions are fulfilled. Previous work carried out in the Department of Human Developmental Biology, Jagiellonian University College of Medicine both in CS and EAE models showed that EC immunization with protein antigen strongly suppressed the induction of the T cell mediated immune response. Further work employing allogeneic skin grafts showed that EC immunization with a protein antigen delays graft rejection. In the current study, It was investigated if EC immunization with a soluble protein antigen via a gauze skin patch could ameliorate the inflammatory response during CIA. It was shown that EC immunization with collagen II \(COLL II\) prior to induction of CIA reduced disease severity in susceptible DBA\/1 mice. The optimal dose of COLL II that inhibits CIA was found to be between 30 and 100 μg\/animal. The decrease in disease severity correlated with milder histological changes, reduced MPO activity in joint tissue, decreased production of pathogenic anti\-type II collagen IgG2a antibody and reduced level of anti\-cyclic citrulinated peptide \(anti CCP\) IgG antibodies. Further experiments showed that suppressive activity can be adoptively transferred by cells isolated from the inguinal and auxiliary lymph node cells and spleens of EC immunized mice. Suppression could b"]

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