Search for: [Abstract = "al health and DM I has been reported as a predisposing factor to oral infections, which in turn exacerbates the progression of systemic diseases. To date, no attempts have been made to characterize the microbiota of particular niches in the oral cavity in DM I patients. Objectives of the work 1. Assessment of the oral microbiota in two different systemic disease states, that is in patients with COVID\-19 hospitalized in an intensive care unit and patients with type 1 diabetes treated with CSII. 2. The determination of optimal sites of oral microbiota sampling concerning bacterio\- and mycobiota and characterisation of the biodiversity of the identified niches. Material and methods In the first part of the study, 56 adult COVID\-19 patients qualified for mechanical ventilation in a Temporary ICU due to pneumonia. Patients oral health assessed using a modified Beck Oral Assessment Score \(BOAS\) \[30\] . Four oral habitats were sampled\: the buccal mucosa, the tongue, buccal dental surface and gingival cervical fluid \(GCF\). The samples were cultured with the use of traditional methods for aerobic and anaerobic bacteria and fungi. After isolation, the microorganisms were identified by MALDI\-TOF MS \(Vitek MS Home bioMérieux\). The second part of the study conducted in 23 adult patients with DM I, aged 18\- 35 years old, treated with IP. The general condition of the"]
