Search for: [Abstract = "The object of the study was Sarcodon imbricatus, a fungus from the Basidiomycota division occurring in spruce forests, covered with strict protection in Poland. In vitro cultures were driven from the hymeneal layer of the sporocarp. A pure line of mycelium cells was obtained in the culture. PCR\-RFLP DNA analysis of the obtained mycelium was performed to confirm genetic purity and compliance with the cells used to initiate the culture. To optimize the culture medium and conditions, various sources of carbon and nitrogen were tested, along with combinations of incubation temperature and initial pH of the medium. Highest increases in biomass were obtained using the optimized Lubiński medium \(Turło et al., 2004\) containing\: fructose \(50g\/l\) and casein hydrolysate \(10g\/l\). The determined optimum culture conditions were 20°C and initial pH of 6.0. The second stage of the study covered chemical analyses of mycelium collected in natural sites and of the mycelium from in vitro cultures. Chemical analyses included the following groups of compounds\: fatty acids, sterols, amino acids, indole compounds, phenolic acids, polysaccharides and mineral compounds. Chromatographic methods \(partition, column and thin\-layer chromatography\) were used in isolation and\/or purification of the analyzed compounds. High\-performance liquid chromatography \(HPLC\) was used for quantitative analysis. Spectral methods \(1H NMR, EI\-MS\) were used for identification of the compounds. The performed chemical analyses identified\: · Eight fatty acids, both saturated and unsaturated in the original sporocarps \(including three unsaturated acids\: oleic acid, linoleic acid and α\-linoleic acid\) and 11 fatty acids in the in vitro culture cells \(including eight unsaturated acids, such as, among others, the α\-linoleic acid\), · Ergosterol, both in natural mycelia and in vitro cells. This compound has been quantitated, isolated and identified using spectral methods \(1H NMR, EI\-MS, UV\-VIS\). Additionally, ergosterol peroxide was identified in the sporocarps. · Presence of proteinogenic amino acids \(endo\- and exogenous\). 14 proteinogenic amino acids were identified and quantitated in the sporocarps \(including eight that are exogenous to humans\). Seven compounds were identified in the in vitro cultures \(including 4 amino acids exogenous to humans\). · Indole derivatives – tryptophan, tryptamine and serotonin were identified in both sporocarp and in vitro mycelium. Additionally, trace amounts of melatonin were found in the sporocarps. Those compounds were identified using spectral methods \(1H NMR, EI\-MS,UV\-VIS\), · Three phenolic acids were found in both the sporocarps and in vitro mycelium \(p\-hydroxybenzoic acid, protocatechuic acid \(3,4\- dihydroxybenzoic acid\) and syryngic acid \(4\-hydroxy\-3,5 dimethoxybenzoic acid\) and identified using spectral methods \(1H NMR, EI\-MS\), · Three polysaccharide fractions \(FOI, FOII and FOIII\) were isolated from the sporocarps and two fractions \(FKI and FKII\) from the in vitro mycelium. Qualitative analysis of the isolated polysaccharide fractions identified galactose and fucose \(FOI, FKI\) and glucose and fucose \(FOII, FKII\). The FOIII fraction from the sporocarps was glucan, · Total percentage content of sugars, percentage content of uronic acids and molecular masses in the isolated fractions, · Qualitative and quantitative composition of the mineral compounds in both in vivo and in vitro material. In the third part of the study, biological activity of selected polysaccharide fractions obtained from the sporocarps and in vitro mycelium was assessed using in vitro tests, covering\: antibacterial activity against five G\(\+\) and G\(\-\) bacterial strains, anti\-viral activity against the HPV\-1 virus and cytotoxicity against animal cancer cells \(melanoma B16 and sarcoma XC\) and human cancer cells \(breast cancer\). Fractions isolated from both the sporocarps and in vitro cultures demonstrated a low level of anti\-bacterial activity."]

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