Search for: [Abstract = "The object of the study was Sarcodon imbricatus, a fungus from the Basidiomycota division occurring in spruce forests, covered with strict protection in Poland. In vitro cultures were driven from the hymeneal layer of the sporocarp. A pure line of mycelium cells was obtained in the culture. PCR\-RFLP DNA analysis of the obtained mycelium was performed to confirm genetic purity and compliance with the cells used to initiate the culture. To optimize the culture medium and conditions, various sources of carbon and nitrogen were tested, along with combinations of incubation temperature and initial pH of the medium. Highest increases in biomass were obtained using the optimized Lubiński medium \(Turło et al., 2004\) containing\: fructose \(50g\/l\) and casein hydrolysate \(10g\/l\). The determined optimum culture conditions were 20°C and initial pH of 6.0. The second stage of the study covered chemical analyses of mycelium collected in natural sites and of the mycelium from in vitro cultures. Chemical analyses included the following groups of compounds\: fatty acids, sterols, amino acids, indole compounds, phenolic acids, polysaccharides and mineral compounds. Chromatographic methods \(partition, column and thin\-layer chromatography\) were used in isolation and\/or purification of the analyzed compounds. High\-performance liquid chromatography \(HPLC\) was used for quantitative analysis. Spectral me"]

Number of results: 1