Search for: [Abstract = "The maximum contents of these compounds in undifferentiating callus cultures amounted to 18.75 mg\/100 g d.w., 2.66 mg\/100 g d.w., 2.16 mg\/100 g d.w. and 1.03 mg\/100 g d.w., respectively. The maximum contents of the lignans in agitating shoot cultures were also interesting from practical point of view and amounted to 92.37 mg\/100 g d.w., 50.69 mg\/100 g d.w., 48.16 mg\/100 g d.w. and 15.77 mg\/100 g d.w., respectively. On the other hand, the maximum lignan amounts in suspension cultures were of another order of magnitude, equaling 21.75 mg\/100 g d.w., 5.54 mg\/100 g d.w., 4.07 mg\/100 g d.w. and 9.42 mg\/100 g d.w., respectively.The maximum total content of lignans obtain in agar shoot\-differentiating callus cultures, amounted to 486.78 mg\/100 g d.w. and was 1.3\- and 3.8\-fold higher than in extracts from fruits and leaves of native plant, respectively, analyzed for comparison.Thus, the studies demonstrated that the established different types of in vitro cultures could be a potential biotechnological source of four lignans under study, especially deoxyschisandrin.Next, analysis of phenolic acids was performed. These compounds have not been studied in Chinese magnolia vine so far. The first common step in biogenesis of lignans and phenolic acids \- shikimic acid pathway was taken into account. The studies in this direction were undertaken because of a wide range of valuable biological properties of these compounds comprising e.g. antiaggregatory, antioxidant, immunostimulating and anticancer activities. The studies demonstrated the effect of growth regulators also on the production of this group of compounds. In addition, they showed that FPA production was the highest in agar undifferentiating\-callus cultures \(max. total content – 78,24 mg\/100 g d.w.\) The maximum FPA content in agar shoot\-differentiating callus cultures was a little lower, amounting to 60.06 mg\/100 g d.w.. The FPA content in agitating shoot cultures \(75.57 mg\/100 g d.w.\). was higher than in suspension cultures characterized by a lower degree of differentiation \(38.40 mg\/100 g d.w.\).The highest TPA contents were obtained in both types of agitating cultures and equaled 213.62 mg\/100 g d.w. in suspension cultures, and 204.15 mg\/100 g d.w. in shoot cultures. The TPA contents in both types of agar cultures were lower but also interesting from practical point of view and amounted to 165.40 mg\/100 g d.w. in callus cultures and 115.64 mg\/100 g d.w. in shoot\-differentiating callus cultures.The maximum obtained FPA content in the biomass extracts \- 78.24 mg\/100 g d.w.\(agar callus cultures\) was 1.4\- and 1.7\-fold higher than in extracts from fruits and leaves of native plant, respectively. The maximum obtained TPA content \- 213.62 mg\/100 g d.w. \(suspension cultures\) was 1.77\- and 15.1\-fold higher than in extracts from the examined organs of native plant, respectively.Chlorogenic acid \(max. content 82.10 mg\/100 g d.w., agar callus cultures, TPA\), protocatechuic acid \(max. content 120,16 mg\/100 g d.w., suspension cultures, TPA\) and syringic acid \(max. content 40,50 mg\/100 g d.w., suspension cultures, TPA\) were the main phenolic acids produced in vitro. The experiments proved that the established in vitro cultures could be a potential biotechnological source of certain phenolic acids. It is noteworthy that this study is the first analysis of phenolic acids in Schisandra chinensis in vitro cultures and in organs of native plant.A distinct part of this work focused on the isolation and purification of lignan fractions and confirmation of the identity of three compounds\: deoxyschisandrin, schisandrin and gomisin A using spectral analysis \(1H NMR spectra\). The mentioned lignans were not isolated earlier from in vitro cultures of this species. The results of this work are not only important from the point of view of basic research but may also find practical applications."]

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