Search for: [Abstract = "The aim of this study was to design the methodology of expansion and differentiation of CD34\+ cells to monocytes\/macrophages. The present study shows that optimal medium for expansion of CD34\+ cells was X\-VIVO 10 supplemented with FBS, SCF, IL\-3, FLT\-3L, TPO and 3\-4 days culture. For differentiation of CD34\+ cells to monocytes\/macrophages was IMDM supplemented with FBS, M\-CSF, SCF, IL\-3, FLT\-3L and culture for 7\-10 days. Using this protocol it was possible to obtain 1000 \- fold increase in number of expanded cells, of which 40\-60% were CD14\+, i.e. 600 \- fold increase in the number of CD14\+ cells. The presence of two subpopulations of monocytes\: CD14\+CD16\- and CD14\+\+CD16\+, which occurred at the ratio of about 2\:1 was observed. They are clearly distinct from known main subpopulations of blood monocytes CD14\+CD16\+ and CD14\+\+. Supplementation of cultures with VD3 increased the percentage of CD14\+CD16\- and decreased the proportion of CD14\+\+CD16\+. Monocytes\/macrophages, mainly CD14\+\+\/16\+ subpopulation secreted IL\-12, but not TNF and IL\-10, induced allo\-MLR \(mainly CD14\+\+\/16\+ subpopulation\), fagocytosed S.aureus \(mainly CD14\+\/16\- subpopulation\), but did not produce intracellular O2\-. Hence, this study shows that it is possible to generate from CD34\+ haematopoietic progenitors two subpopulations of human monocytes. They are clearly distinct from those present in the peripheral blood im"]

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