Search for: [Abstract = "The aim of the study was to assess the effect of R115777 \(farnesyltransferase inhibitor designed to disrupt Ras protein signaling\) on the proliferation and apoptosis of melanoma cell lines derived from different stages of cancer progression. The attempts of elucidation of the cell response mechanism for applied inhibitor – especially in the context of apoptosis induction, as well as pointing the potential molecular targets for drugs that by synergistic action with R115777 could increase therapy effectiveness were contemporarily undertaken. The R115777 evidently inhibited proliferation rate \(BrdU, crystalline violet assay\) and induced apoptosis \(caspase\-3 activation assay, TUNEL\) in the case of melanoma cell lines derived from later stages of disease progression \(vertical growth phase, metastasis\) what was initiated in Ras inactivation independent mode. The cytotoxic effect was relevant to molecular disorders frequently observed in the later phases of melanoma, such as B\-Raf activating mutation and high initial activation level of PI3K\/Akt pathway determined by PTEN deletion\/mutation. In that context the strong enhancement of R115777 pro\-apoptotic activity by chaperone HSP90 inhibitor \(17AAG\) as well as by PI3K inhibitor \(Ly294002\) argues for further studies in order to create a new treatment regimens allowing for effective elimination of cancer cells in case of advanced melanom"]

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