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Search for: [Abstract = "Splenectomy is an approved method of some hematological disorders as well as spleen rupture treatment. However, asplenia predisposes to severe bacterial infections. After splenectomy, numerous immunological functions of B cells, T cells and phagocyticcells are impaired. Impairment of IgM\-dependent humoral immunity is thought to be the main cause of postsplenectomy infections and may be secondary to disordered peripheral B cell development. However, the role of spleen in B cell homeostasis, especially playing crucial role in humoral immunity memory B cells remains elusive.The aim of this study was to determine if the spleen plays a role in the regulation of circulationg blood cellular composition and to determine, if splenectomy affects circulationg blood composition, especially of B cell subpopulations.Blood morphology and numbers of B cell subpopulations were analyzed in blood samples taken form splenic and peripheral vein during elective laparoscopic splenectomy. The same analyses were repeated 6 months after splenectomy. Using flow cytometry, the counts of the following B cell subpopulations were determined\: naïve B cells \(CD19\+CD27\-\), class\-switched memory B cells \(CD19\+CD27\+IgD\-IgM\-\), IgM memory B cells \(CD19\+CD27\+ IgD\+IgM\+\), transitional B cells \(CD19\+CD38\+\+IgMhigh\), class\-switched plasmablasts \(CD19\+\/CD38\+\+\+\/IgM\-\) and activated B cells \(CD19\+\/CD21low\/CD38low\).The numbers of circulating erythrocytes, platelets, leukocytes and neutrophiles were decreased in splenic blood comparing to peripheral blood. Lymphocyte count was similar in splenic and peripheral blood and lymphocyte B count was increased in splenic blood. The number of IgM and class\-switched memory B cells was incresed in splenic blood. The number of class\-switched plasmablasts, naïve, transitional and activated B cells was similar in splenic and peripheral blood.After splenectomy the number of circulating erythrocytes and platelets in peripheral blood was increased, the number of leukocytes was not changed, and the number of neutrophiles was decreased. Also, total lymphocyte and B cell counts were increased after splenectomy.The increase in B cell count was associated with increase of naïve and transitional B cell numbers. The numbers of memory B cells, class\-switched plasmablasts and activated B cells remained unchanged after splenectomy.These results suggest that the spleen continuously releases memory B cells into bloodstream. Splenectomy seems to affect the peripheral development of B cells, although it do not affect the numbers of circulating memory B cells. The clinical significance of these observations in regard to the patophysiology of postsplenectomy infections remains not entirely clear."]

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