Search for: [Abstract = "Splenectomy is an approved method of some hematological disorders as well as spleen rupture treatment. However, asplenia predisposes to severe bacterial infections. After splenectomy, numerous immunological functions of B cells, T cells and phagocyticcells are impaired. Impairment of IgM\-dependent humoral immunity is thought to be the main cause of postsplenectomy infections and may be secondary to disordered peripheral B cell development. However, the role of spleen in B cell homeostasis, especially playing crucial role in humoral immunity memory B cells remains elusive.The aim of this study was to determine if the spleen plays a role in the regulation of circulationg blood cellular composition and to determine, if splenectomy affects circulationg blood composition, especially of B cell subpopulations.Blood morphology and numbers of B cell subpopulations were analyzed in blood samples taken form splenic and peripheral vein during elective laparoscopic splenectomy. The same analyses were repeated 6 months after splenectomy. Using flow cytometry, the counts of the following B cell subpopulations were determined\: naïve B cells \(CD19\+CD27\-\), class\-switched memory B cells \(CD19\+CD27\+IgD\-IgM\-\), IgM memory B cells \(CD19\+CD27\+ IgD\+IgM\+\), transitional B cells \(CD19\+CD38\+\+IgMhigh\), class\-switched plasmablasts \(CD19\+\/CD38\+\+\+\/IgM\-\) and activated B cells \(CD19\+\/CD21low\/CD38low\).The numbers"]

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