Search for: [Abstract = "Schisandra chinensis \- Chinese magnolia vine \(Schisandraceae\) is a dioecious vine native to some regions of Southeastern Asia. It is a new pharmacopoeial species in the European medicine from 2008 and also in Polish medicine \(from 2009\). Schisandra fruits are a rich source of lignans, dibenzocyclooctadiene derivatives. These compounds are highly valued mostly for their adaptogenic, hepatoprotective, antioxidant and anticancer properties.This work aimed to establish Schisandra chinensis in vitro cultures and to analyze two groups of therapeutically important compounds\: lignans and phenolic acids. The further goal of these studies was to propose in vitro cultures as a potential source of the abovementioned groups of compounds.In vitro cultures with different degree of differentiation \(shoot\-differentiating callus cultures and undifferentiating callus cultures\) were successfully established from leaf buds of female Schisandra plants. At the next stage of research, culture conditions were optimized by testing several variants of the Murashige and Skoog \(MS\) medium differing in the contents of plant growth regulators\: the cytokinin BAP \(6\-benzyloaminopurine\) and the auxin NAA \(α\-naphthaleneacetic acid\), in the concentration range from 0.1 to 3.0 mg\/l, and by examination of different types of in vitro cultures\: agar \(shoot\-differentiating callus and callus\) cultures and agitating \(shoot and suspension\) cultures. The next stage of research involved HPLC determination of the composition and contents of four main lignans of Chinese magnolia vine and eleven phenolic acids, cinnamic and benzoic acid derivatives and the parent compound of one of the subgroups of these compounds \- cinnamic acid. Free phenolic acids \- FPA and total phenolic acids – TPA, free and bound phenolic acids \(obtained after acid hydrolysis\), were estimated.The established shoot\-differentiating callus cultures were characterized by a distinct capability of deoxyschisandrin production. The contents of schisandrin and gomisin A were also high while γ\-schisandrin amounts were lower. The studies demonstrated the effect of plant growth regulators on the lignan production. In addition, it was shown that the cultures at the higher level of differentiation produced many times greater amounts of these compounds than undifferentiating callus cultures.The maximum contents of lignans equaling 308.51 mg\/100 g d.w. for deoxyschisandrin, 86.41 mg\/100 g d.w. for gomisin A, 75.54 mg\/100 g d.w. for schisandrin and 22.09 mg\/100 g d.w. for γ\-\-schisandrin, were obtained in agar shoot\-differentiating callus."]

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