Search for: [Abstract = "PPAR ligands at concentrations used in these experiments \(ciglitazon \- 5, 10, 15 μM, fenofibrat \- 5, 10, 15 μM, karbacyklin \- 0.1, 1.0, 5.0 μM, linoleic acid \- 15, 20, 30 μM and 9\-cis\-retinoic acid \- 0.1, 0.3, 1.0\) decreased proliferation of melanoma cells, however, these changes were in long time reversible in our experimental setup. None of PPARs ligands under investigation induced apoptosis in studied cell lines. The results of the present work suggest that PPARα may play an important part in the control of melanoma growth. This was evidenced by the change in cell proliferation rate and in the secretion of MMPs, VEGF and, importantly, MIA and NFκB as a result of activation of this receptor by a specific ligand. The proteins MIA and NFκB are prognostic markers of melanoma progression in vivo. PPARγ, despite its influence on melanoma cell proliferation, did not prove to be a universal „controller” of the mechanisms significant for neoplastic growth. It was also demonstrated that PPARβ\/δ, in the cell lines examined in this study, played a role of a “proliferation controller”. Since the study showed that the increased expression of PPARδ\/β protein was correlated with the elevated malignancy of the cell line, it can be hypothesized that the receptor and its ligand carbacycline decreased growth potential of melanoma cells. The obtained results indicate an important function of PPARs in the control of proliferation both directly by influencing certain cell cycle proteins and via antagonizing NFκB action. They are also a good point for continuation of in vivo studies on the role of PPAR in biology of the melanoma."]

Number of results: 0