Search for: [Abstract = "Negative selection experiments with monoclonal antibodies and rabbit complement revealed that these cells are TCRαβ\+ CD4\+ lymphocytes \(contasuppressor T cell, Tcs\). Experiments in an active immunization and adoptive transfer of CS employing three non\-crossreacting antigens TNP\-Ig, OX\-Ig and OVA showed that contrasuppression induced via epicutaneous immunization with an antigen and LPS is antigen specific. In the final section of this dissertation, the mechanism of contrasuppression induced via epicutaneous application of TNP\-Ig with LPS is discussed. Four different approaches were used\: measurement of cytokine production in vitro, induction of Tcs cells in IL\-6\-\/\-, IL\-12\-\/\-, IFN\-γ\-\/\- and control mice \(adoptive transfer of CS\), neutralization of IFN\-γ at the time of induction of T effector, Tcs and Ts cells \(adoptive transfer of CS\) and replacement of Tcs by rIL\-12 \(adoptive transfer of CS\). These experiments showed that contrasuppression is IFN\-γ and IL\-12 dependent, whereas IFN\-γ is required for induction of Tcs cells that activate antigen presenting cells to release IL\-12, which protects T effector cells from the action of Ts cells. This dissertation describes a novel method to reverse skin induced suppression via epicutaneous immunization with an antigen and PAMP. Because of its effectiveness, ease of induction and non invasiveness, this route of immunization may be effective for new vaccines and anti\-cancer therapy."]

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