Search for: [Abstract = "In this study, two original bioanalytical methods for quantification of eicosanoids biosynthesised from arachidonic acid \(AA\) via different metabolic pathways were developed and validated using UPLC\-MS\/MS technique. The methods allowed to assess the eicosanoid profile in selected animal models of endothelial dysfunction and its changes upon pharmacological modulation of vascular function.In NO\-deficient mice, the biosynthesis of eicosanoids formed by cyclooxygenase \(COX\) was elevated. MNA treatment improved the vessel function by activation of COX\-independent mechanisms.In murine model of hypertension, angiotensin II infusion increased the biosynthesis of CYP450\- derived 20\-HETE. The inhibition of thrombin activity by dabigatran improved the function of endothelium by sustaining the NO bioavailability and reducing the 20\-HETE production.The development of 4T1 breast cancer was associated with the elevated biosynthesis of eicosanoids formed by COX and lipoxygenase \(LOX\) and with the reduced production of CYP450\-derived AA metabolites. The inhibition of NO synthase increased the biosynthesis of epoxyeicosatrienoic acids \(EET\) and 20\-HETE, which probably supported the disease progression under NO\-deficiency.The obtained results show the heterogeneity of eicosanoid profile in investigated murine models of endothelial dysfunction and the pharmacological modulation of vascular functi"]

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