Search for: [Abstract = "Ginkgo biloba callus cultures were established from male and female leaves explants. Suspension cultures were derived from callus cultures. Murashige\-Skoog medium supplemented with Picloram \(4 mg\/l\) and BAP \(2 mg\/l\) was the best medium for further in vitro cultures cultivation. The contents of ginkgotoxin and pyridoxamine were analysed in leaves, seeds and in vitro cultures. The comparison of ginkgotoxin and pyridoxamine contents in male and female leaves collected every week in July 2005 revealed that in the middle of July the amount of ginkgotoxin is the highest.The contents of monomeric flavonoids and phenolic acids after hydrolysis of glycosides and biflavonoids were investigated by HPLC method. The results suggest that G. biloba in vitro cultures can accumulate these compounds, but only in small amounts. The phytochemical analysis of methanol extracts from callus cultures \(two lines\) resulted in the isolation of 12 compounds which were identified as β\-sitosterol, 7\-oxo\-β\-sitosterol, 7α\-hydroxy\- and 7β\-hydroxy\-β\-sitosterol, 3\-O\-β\-glucopyranoside\-β\-sitosterol, p\-hydroxycinnamic alkohol, esculetin, ethyl p\-hydroxybenzoate, p\-hydroxybenzoic acid, caffeic acid, protocatechuic acid, 6\-hydroxykynurenic acid. The occurence of these compounds in in vitro cultures of the plant was been shown for the first time."]

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