Search for: [Abstract = "Gene expression modulation with genome editing \(GE\) tools and RNA interference technology is widely used in tumor research. GE utilizes clustered regularly interspaced short palindromic repeats \(CRISPR\)\/Cas9 or transcription activator\-like effector nucleases \(TALEN\) tools to generate precise double\-strand breaks in the DNA structure. Induced repair mechanisms may lead to gene knockout. shRNA leads to the gene knockdown as the result of the interaction with the targeted transcript. In the current study, three different methods for SNAI1 knockout or knockdown in rhabdomyosarcoma \(RMS\) cells were compared The aims of the study were to design CRISPR\/Cas9 and TALEN systems targeting SNAI1 and to compare their effectiveness and biological effects with shRNA. Therefore, CRISPR\/Cas9, TALEN and shRNA tools were introduced separately to the cells. Next, the genome sequence, transcript levels, and protein expression levels of SNAI1 were evaluated. The biological effects of SNAI1 expression modulation were accompanied by changes in the morphology of the cells and modulation of the expression of myogenic factors \(MYOD, MYOG, MHC, MSTN and MEF2A\) and HDAC1. We confirmed the similar effectiveness of the tested methods. Nevertheless, the low efficiency of the GE tools was a limiting factor in obtaining biallelic gene knockouts. To conclude, we established and characterized thr"]

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