Szukana fraza: [Abstrakt = "D\-hydantoinase is a commercially enzyme used for the preparation of optically pure D\-amino acids from D,L\-5\-monosubstituted hydantoins via hydantoinase method. It catalyzes the reversible hydrolytic ring cleavage of hydantoin to yield N\-carbamoyl\-D\-amino acids, which can be further chemically or enzymatically converted to D\-amino acids. D\-amino acids are important chiral building blocks for biologically active compounds including\: pesticides, peptidomimetics and semisynthetic antibiotics. Also, several enantiomers hydroxy\- or carboxy\- derivatives of phenylglycine or phenylalanine have been described as potent agonists or antagonists at glutamate receptors of the central nervous system. In recent years industrial demand for D\-hydantoinases and N\-carbamoylases with enhanced activity and substrates specificity have been growing rapidly. Hence, up to now natural habitats of the enzymes involved in hydantoinase method have been intensively screened for the presence of microbes having high levels of D\-hydantoinase or N\-carbamoylase activity. Moreover, the introduction of D\-hydantoinase and N\-carbamoylase gene into Escherichia coli by employing recombinant DNA techniques and next their expression or co\-expression lead to enhanced activity, increased enantioselectivity and enlarged substrate specificity of examined enzymes. Additionally, the immobilization technique offers many advanta"]

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