Search for: [Abstract = "CA, NTA, FTIR and evaluated for cytotoxicity using the A549 cell line \(ATCC CCL\-185\) and bacterial adhesion using reference strains of S. aureus \(ATCC® 25923™\) and E. coli \(ATCC ® 25922™\). The evaluation of gentamicin cytotoxicity was performed using the MTT protocol. Optical microscopy, SEM and the LIVE\/DEAD method using fluorescence microscopy were used to image the cytotoxic effect. Results Every patient\-provided tracheostomy tube was culture\-positive. In total, 58 isolates \(10 species\) were isolated and identified. In this study, bacteria that are typically considered to be part of the physiological microbiota were removed, while bacteria from the PPM \(potentially pathogenic microorganisms\) group were included in further investigation. 16 isolates of Staphylococcus aureus were examined from the Gram\-positive PPM \(found in 35.5% of patients\). As many as 76.2% \(32 isolates\) of the identified bacteria in the case of Gram\-negative PPM referred to the order Enterobacterales \(family Enterobacteriaceae\), followed by nonfermenting Gram\-negative bacteria \(NFGNB\), which included Pseudomonas aeruginosa \(n = 5, identified in 11.9%\), Stenotrophomonas maltophilia \(n = 4, in 9.5% of patients\), and Acinetobacter baumani \(n=1, 2,4% of patients\). The most frequently found Enterobacterales bacterium was Klebsiella pneumoniae \(n = 10, found in 23.8% of patients\), followed by E"]
