Search for: [Abstract = "Apart from nitric oxide and carbon monoxide, hydrogen sulphide is another inorganic gaseous mediator. It is produced endogenously in the liver, kidneys, brain and many other human and mammalian tissues. The aim of the study were to determine the production of H2S in various tissues and cell cultures by examining the activity of the enzymes responsible for its formation and metabolism \(CBS, CTH, MPST, TST\) and to determine H2S levels in healthy and inflammatory tissues. A method of determination the activity of the CBS\/CTH tandem in the presence of homoserine \(CBS substrate\) and cystathionine \(CTH substrate\) was developed. For the analysis of changes in cystathionine concentration the RP\-HPLC method was modified. The modification also allowed for determination, during one separation, of the level GSH, GSSG, CSH, CSSC and cystathionine. The research using the developed methods allowed for defining the capabilities of different tissues \(the liver, kidneys, brain\) and cancer cells in the production of H2S and explained the role of H2S in the inflammation caused by stress, associated with damage to the gastric mucosa."]

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