Search for: [Abstract = "Antiphospholipid antibodies are directed against proteins \(mostly B2\- glycoprotein I and prothrombin\) forming complexes with negatively charged phospholipids. Association of antiphospholipid antibodies \(LA and\/or aCL and\/or aB2GPI\) with characteristic clinical symptoms \(thrombosis and obstretical complications\) forms the criteria of antiphospholipid syndrome. The presence of antiphospholipid antibodies is detected by means of two independent methods, coagulometric \(LA\) and immunoenzymatic \(aCL and\/or aB2GPI\). According to guidelines published in 2006, aPL antibodies are considered as classification criteria for APS, as long as they are determined using standardized methods, i.e. based on recognized calibrators for aCL and aB2GPI, and according to ISTH Standardization Subcommittee in case of LA. The purpose of the present study was to assess the usefulness of monoclonal antibodies \(23H9 and 28F4 for LA, and HCAL and EY2C9 for aCL and afB2GPI, respectively\) as standards for antiphospholipid antibodies detection methods. The interaction between mouse antibodies against human B2\-glycoprotein I \(23H9\) and human prothrombin \(28F4\), possessing the properties of lupus anticoagulant was analyzed in 24 human plasma samples. B2GPI\-dependent LA effect observed in phospholipid\-dependent coagulation assays differed markedly from LA associated with the presence of antibod"]

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