Search for: [Abstract = "2α were detected in BaP\-activated cells. The addition of EPA, DHA, and RvD1 to 3T3\-L1 cells, both before and after differentiation, significantly reduced the number of isoprostanes, which indicates the anti\-inflammatory and antioxidant properties of n\-3 fatty acids and their derivatives. The study confirming the properties of fatty acids was also described in another experiment, where adipocytes were incubated with 25 μmol of EPA or DHA and 10 μmol of metformin \(MET\), and 2.5 μmol of lovastatin \(LOVA\). 3T3\-L1 cells were activated with tumor necrosis factor TNF\-α. Next, the levels of cyclooxygenase 2 \(COX\-2\) and glucose transporter \(GLUT\-4\) proteins were examined by the Western Blot technique. The highest level of COX\-2 was demonstrated in TNF\-α activated adipocytes, while in 3T3\-L1 cells, the level of GLUT\-4 protein was the lowest after activation with the inflammatory factor. The addition of metformin significantly increased the level of GLUT\-4. Incubation of cells with fatty acids and LOVA also increased GLUT4 levels, indicating the tested compounds' synergistic role in regulating adipocyte metabolism and preventing diabetes. In adipocytes incubated with TNF\-α, the Ptgs2 and Pla2g4a gene was overexpressed, while for the Pparg gene, a statistically significant reduction was noted. Pparg overexpression was observed in cells incubated with MET, LOVA, EPA, and DHA. The obtaine"]

Number of results: 1