Search for: [Abstract = "tically significant. To determine if a data set is wellmodeled by a normal distribution, the chi\-square test of independence has been applied. To define statistical significance, the Mann–Whitney U test and t test were used, as appropriate. The third paper shows the results of molecular analysis performed on 10 patients with WDTC and 14 patiens with FA. Age, sex, presence of Hashimoto disease, diameter of the tumor, presence of FNAB suspicious for Hurthle cell neoplasm and US risk factors according to EU\-TIRADS were compared between both groups. The specimens of tumor and macroscopiclly normal thyroid tissue were excised in the operating theatre by experienced pathologist. The specimens were placed immediately into RNAlater® and stored at −80°C until they were ready for RNA extraction All blood samples were taken in the operating theatre, before a skin incision was done. Tumor’s RNA was extracted from 50–100 mg of disrupted tissue in TissueLyser LT \(Qiagen, Hilden, Germany\). RNA from 0.5ml plasma was extracted using total RNA protocol of RNAzol Small RNA was used to generated NGS library. For quality purpose we remove all reads with length below 15 nucleotides, and without adapter. As a next step new generation sequencing has been performed on NextSeq \(llumina, San Diego, CA, USA\). Results have been saved on the local server. Quality of reads has been assessed u"]
