Search for: [Abstract = "f this enzyme has been characterized only on the activity of doxorubicin and daunorubicin. The aim of the research carried out as part of the doctoral thesis was to determine the importance of the CBRl enzyme in the resistance of cancer cells to various anthracyclines, by determining the susceptibility of anthracyclines to biotransformation with the participation of CBRl, expanding the state of knowledge about CBRl\-dependent mechanisms involved in anthracycline resistance and demonstrating the relationship between CBRl\-dependent metabolism, and ABCB I\-dependent drug efflux from cells. In the course of the study, the biotransformation of eight anthracyclines was examined by determining the parameters\: t112 and Clint\- In silico, the value of the partial negative charges of carbonyl oxygen of anthracyclines was calculated and their molecular docking to the CBRl active site was performed. The observed differences in the process of individual anthracyclines docking to the active site of the enzyme proved that the presence of a hydroxyl group in the side chain plays a significant role in the rate of biotransformation. Further experiments were carried out usmg genetically modified A549 non\-small cell lung cancer cells overexpressing CBR\], which showed that CBRl differently affects the activity of individual anthracyclines. It was confirmed that the activit"]

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