Szukana fraza: [Abstrakt = "Apoptosis, called programmed cell death, is one of the main physiological proceses that maintain tissue homeostasis. Apoptosis dysfunction can be observed in many pathological conditions like inflammation, cancer, etc. Cytokines, reactive oxygen species, hypoxia can initiate programmed cell death. Pathogen and commensal bacteria are in close contact with epithelial tissue and they can influence apoptotic pathways. The Lactobacillus species that are potential probiotic strains can also be important in maintaining tissue balance. They can secrete several different substances that can act on programmed cell death. The aim of this doctoral dissertation was the effect of different Lactobacillus strains on epithelial cells apoptosis. Apoptosis of cell line HT\-29, CaCo\-2, A431 and mouse primary colonocytes was tested. Potentially probiotic and hydrogen peroxide producing Lactobacillus strains and their supernatants have been chosen for these experiments. The level of activated caspases and cell membrane damage has been estimated by flow cytometry. Fluorescent FAM\-VAD\-FMK caspase inhibitor and propidium iodide have been used to distinguish apoptosis from necrosis. Apoptotic pathway was confirmed by DNA ladder electrophoresis. Changes of the actin filaments structure have been observed after Lactobacilli incubation have been performed. Among many Lactobacillus strains chosen three diffe"]

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